Identification of divergent isolates of cherry latent virus 1 in Greek sweet cherry orchards

Chrysoula G Orfanidou (1) , Asimina Katsiani (1) , Thierry Candresse (2) , Armelle Marais (2) , Theodora Gkremotsi (1) , Pavlina Drogoudi (3) , Konstantinos Kazantzis (3) , Nikolaos I Katis (1) , Varvara I Maliogka (1)

1 Aristotle University of Thessaloniki
2 BFP - Biologie du fruit et pathologie
3 HAO Demeter - Hellenic Agricultural Organization Demeter

In this study, samples collected from eight sweet cherry trees in northern Greece were analyzed by high-throughput sequencing for the presence of viruses. Bioinformatic analysis revealed the presence of divergent isolates of cherry latent virus 1 (CLV-1), a recently identified trichovirus in a sweet cherry accession imported into the USA from the Republic of Georgia. The complete genome sequences of seven CLV-1 isolates were determined, and phylogenetic analysis indicated that they belonged to a separate clade from the previously characterized Georgian isolate. A small-scale survey confirmed the presence of CLV-1 in 47 out of 151 sweet cherry samples tested, and partial sequencing of 15 isolates showed a high degree of nucleotide sequence similarity among them.

Mots clés: Greece trichovirus; cherry latent virus 1; high-throughput sequencing; genetic diversity; virome

Domaines: Sciences du Vivant [q-bio]; Phytopathologie et phytopharmacie

Chrysoula G Orfanidou, Asimina Katsiani, Thierry Candresse, Armelle Marais, Theodora Gkremotsi, et al.. Identification of divergent isolates of cherry latent virus 1 in Greek sweet cherry orchards. Archives of Virology, 2023, 168 (10), pp.243. ⟨10.1007/s00705-023-05875-7⟩. ⟨hal-04228187⟩