Identification and characterization of a female gametophyte defect in sdk1-7 +/- abi3-6 +/- heterozygotes of Arabidopsis thaliana

Subramanian Sankaranarayanan (1, 2) , Muhammad Jamshed (1, 3) , Frédéric Delmas (4) , Edward Yeung (1) , Marcus Samuel (1)

1 University of Calgary
2 Purdue University [West Lafayette]
3 Frontier Agri-Science
4 BFP - Biologie du fruit et pathologie

Successful reproduction in angiosperms is dependent on the highly synchronous development of their male and female gametophytes and the ensuing fusion of the gametes from these reproductive tissue types. When crossing a T-DNA insertion line sdk1-7-/-(Salk_024564), one of the S-domain receptor kinases involved in ABA responses with a fast neutron deletion line abi3-6-/-, the F1 heterozygotes (sdk1-7+/-abi3-6 +/-) displayed 50% ovule abortion suggesting a likely gametophytic defects. We identified and characterized an early stage female gametophyte developmental defect in the heterozygous mutant ovules. Recombination frequency analysis of the F2 progenies from selfed heterozygotes revealed a possible pseudo-linkage of sdk1-7 and abi3-6 suggesting a reciprocal translocation event in the heterozygote. Our study emphasizes the importance of robust analysis to distinguish gametophytic defect phenotypes caused by genetic interactions and that resulting from possible chromosomal translocation events.

Mots clés: B3 domain transcription factor; Female gametophyte (FG); S-domain receptor kinase; T-DNA insertion; abi3; fast neutron deletion; heterozygote; pseudo-linkage; reciprocal translocation

Domaines: Sciences du Vivant [q-bio]; Biologie végétale

Subramanian Sankaranarayanan, Muhammad Jamshed, Frédéric Delmas, Edward Yeung, Marcus Samuel. Identification and characterization of a female gametophyte defect in sdk1-7 +/- abi3-6 +/- heterozygotes of Arabidopsis thaliana. Plant Signaling and Behavior, 2020, 15 (8), pp.1780038. ⟨10.1080/15592324.2020.1780038⟩. ⟨hal-03454328⟩