Mapping the phytoplasma chromosome

Sylvie Malembic-Maher (1) , Patricia Carle (1)

1 BFP - Biologie du fruit et pathologie

Physical and genetic mapping of the phytoplasma chromosome can be a useful tool in a genome sequencing project in order to assemble the in silico-predicted contigs robustly. Mapping consists of four distinct steps: preparation of phytoplasma chromosomes from infected plants, single- and double-digestion of chromosomes with rare-cutting restriction enzymes, separation of large DNA fragments by pulsed-field gel electrophoresis, and hybridization with various genetic markers. Materials and methods needed for each step are described and the technique is illustrated using the flavescence doree phytoplasma genome map as an example.

Mots clés: Chromosome Mapping/ methods; phytoplasma; genetic map; physical map; pulsed-field gel electrophoresis; restriction endonucleases; southern blot

Mots clés: flavescence dorée; mollicutes

Domaines: Phytopathologie et phytopharmacie

Sylvie Malembic-Maher, Patricia Carle. Mapping the phytoplasma chromosome. Phytoplasma: Methods and Protocols, 938, Springer Science+Business Media, 2013, Methods in Molecular Biology, 978-1-62703-088-5 978-1-62703-089-2. ⟨10.1007/978-1-62703-089-2_34⟩. ⟨hal-02804649⟩