Viral Double-Stranded RNAs (dsRNAs) from Plants: Alternative Nucleic Acid Substrates for High-Throughput Sequencing.

Armelle Marais (1) , Chantal Faure (1) , Bernard Bergey (1) , Thierry T. Candresse (1)

1 BFP - Biologie du fruit et pathologie

High-throughput sequencing (or next-generation sequencing-NGS) is an emerging technology that allows the detection of plant viruses without any prior knowledge. Various sequencing techniques and various templates can be used as substrate for NGS. This chapter describes an optimized protocol for the extraction of double-stranded RNAs (dsRNAs) from a wide range of plants and for their random amplification prior to NGS sequencing.

Mots clés: Diagnostic; Double-stranded (ds) RNA; High-throughput sequencing; NGS; Virome

Mots clés: Metagenomics

Domaines: Phytopathologie et phytopharmacie; Virologie

Armelle Marais, Chantal Faure, Bernard Bergey, Thierry T. Candresse. Viral Double-Stranded RNAs (dsRNAs) from Plants: Alternative Nucleic Acid Substrates for High-Throughput Sequencing.. Viral Metagenomics: Methods and Protocols, 1746, Springer Science + Business Media LLC, 2018, Methods in Molecular Biology, 978-1-4939-7683-6_4. ⟨10.1007/978-1-4939-7683-6_4⟩. ⟨hal-02789974⟩