Development of EST-SSR markers from an inner bark cDNA library of Fagus crenata (Fagaceae)

Saneyoshi Ueno (1, 2, 3) , Yuriko Taguchi (2) , Nobuhiro Tomaru (4) , Yoshihiko Tsumura (2)

1 BioGeCo - Biodiversité, Gènes & Communautés
2 FFPRI - Forestry and Forest Products Research Institute
3 Cirad - Centre de Coopération Internationale en Recherche Agronomique pour le Développement
4 Graduate School of Bioagricultural Sciences

Fagus crenata Blume is widely distributed throughout Japanese cool-temperate deciduous broad-leaved forests, but there are two divergent groups of populations in areas with contrasting winter climates separated by Japan’s Central Mountain Range. To facilitate investigations of adaptive genetic differentiation of the species using potentially functional genes, we have collected Expressed Sequence Tags and developed Simple Sequence Repeat markers using a cDNA library constructed from cambium and surrounding tissues. In total, 270 primer pairs were designed, and 87 of the corresponding loci showed polymorphism in 16 individuals, with 2–21 alleles per locus and expected heterozygosities ranging from 0.06 to 0.97. EST-SSR markers developed in the present study will be useful for genomic analyses of F. crenata populations.

Mots clés: FAGUS CRENATA; ADAPTATION; EXPRESSED SEQUENCE TAG; JAPANESE BEECH; CHENE; HETRE

Mots clés: QUERCUS; MICROSATELLITE; ADAPTATION GENETIQUE

Domaines: Génétique

Saneyoshi Ueno, Yuriko Taguchi, Nobuhiro Tomaru, Yoshihiko Tsumura. Development of EST-SSR markers from an inner bark cDNA library of Fagus crenata (Fagaceae). Conservation Genetics, 2009, 10 (5), pp.1477-1485. ⟨10.1007/s10592-008-9764-1⟩. ⟨hal-02662177⟩