A fluorometric assay for trehalose in the picomole range

Petronia Carillo (1) , Regina Feil (2) , Yves Y. Gibon (3, 2) , Namiko Satoh-Nagasawa (4) , David Jackson , Oliver E Bläsing (2) , Mark Stitt (2) , John Edward Lunn (2)

1 Seconda Università degli Studi di Napoli = Second University of Naples
2 MPI-MP - Max Planck Institute of Molecular Plant Physiology
3 BFP - Biologie du fruit et pathologie
4 CSHL - Cold Spring Harbor Laboratory

The fluorometric assay is highly specific for trehalose and sensitive enough to measure the trehalose content of very small amounts of plant tissue. Chilling induced a 2-fold accumulation of trehalose in A. thaliana rosettes, but the levels were too low to make a substantial quantitative contribution to osmoregulation. Trehalose is unlikely to function as a signal of sucrose status. The abnormal inflorescence branching phenotype of the maize ramosa3 mutant might be linked to a decrease in trehalose levels in the inflorescence primordia or a downward shift in the trehalose:Tre6P ratio.

Mots clés: arabidopsis thaliana; trehalase; trahalose; zea mays

Mots clés: ramosa3

Domaines: Biologie végétale

Petronia Carillo, Regina Feil, Yves Y. Gibon, Namiko Satoh-Nagasawa, David Jackson, et al.. A fluorometric assay for trehalose in the picomole range. Plant Methods, 2013, 9 (1), pp.21. ⟨10.1186/1746-4811-9-21⟩. ⟨hal-02645455⟩