Complete genomic sequence of barley (Hordeum vulgare) endornavirus (HvEV) determined by next-generation sequencing.

Thierry T. Candresse (1) , Armelle Marais (1) , Roberto Sorrentino (2) , Chantal Faure (1) , Sébastien Theil (1) , Valérie Cadot (3) , Mathieu Rolland (3) , Julie Villemot (3) , Frank Rabenstein (4)

1 BFP - Biologie du fruit et pathologie
2 Dipartimento di Agraria
3 GEVES - Groupe d'Etude et de Contrôle des Variétés et des Semences
4 JKI - Julius Kühn-Institut

Endornaviruses are unusual plant-, fungus- and oomycete-infecting viruses with a large, ca 14- to 17-kb linear double-stranded RNA (dsRNA) genome and a persistent lifestyle. The complete genome sequence of an endornavirus from the barley (Hordeum vulgare) Nerz variety was determined from paired Illumina MySeq reads derived from purified dsRNAs. The genome is 14,243 nt long, with 5' and 3' non-coding regions of 207 and 47 nt, respectively. It encodes a single large protein of 4663 amino acids that carries conserved motifs for a methyltransferase, a helicase and an RNA-dependent RNA polymerase. The sequence of Hordeum vulgare endornavirus (HvEV) carries all the hallmarks of a typical member of the genus Endornavirus, with the exception of an UDP-glycosyltransferase motif observed in many, but not all, endornaviral genomes.

Domaines: Virologie; Biologie végétale

Thierry T. Candresse, Armelle Marais, Roberto Sorrentino, Chantal Faure, Sébastien Theil, et al.. Complete genomic sequence of barley (Hordeum vulgare) endornavirus (HvEV) determined by next-generation sequencing.. Archives of Virology, 2016, 161 (3), pp.741-743. ⟨10.1007/s00705-015-2709-3⟩. ⟨hal-02636220⟩