Molecular characterization of divergent grapevine Pinot gris virus isolates and their detection in Slovak and Czech grapevines.

Miroslav Glasa (1) , Lukáš Predajňa (1) , Petr Komínek (2) , Alžbeta Nagyová (1) , Thierry T. Candresse (3) , Antonio Olmos (4)

1 SAS - Slovak Academy of Sciences
2 Crop research institute
3 BFP - Biologie du fruit et pathologie
4 IVIA - Instituto Valenciano de Investigaciones Agrarias - Institut Valencià d'Investigacions Agraries - Valencian Institute for agricultural Research

Analysis of complete genome sequences of three Slovak isolates of grapevine Pinot gris virus (GPGV) showed their low heterogeneity (reaching 1.7 %) and a close relationship to the Italian NC_015782 isolate (4.2-4.5 % divergence). Comparison of Slovak and Italian isolates revealed an unusual accumulation of 21 indel mutations in ORF1, resulting in a localized high divergence in the encoded amino acid sequences. An elevated divergence in the 5' extremity of the GPGV genomes is suggestive of a recombination between Slovak isolates and grapevine berry inner necrosis virus. RT-PCR allowed the frequent detection of closely related GPGV isolates in grapevines from Slovakia and the Czech Republic.

Mots clés: Trichovirus; Next-generation sequencing; Diversity; Detection

Domaines: Autre [q-bio.OT]

Miroslav Glasa, Lukáš Predajňa, Petr Komínek, Alžbeta Nagyová, Thierry T. Candresse, et al.. Molecular characterization of divergent grapevine Pinot gris virus isolates and their detection in Slovak and Czech grapevines.. Archives of Virology, 2014, 159 (8), pp.2103-2107. ⟨10.1007/s00705-014-2031-5⟩. ⟨hal-02635271⟩