Development of SNP markers for the African timber species Nauclea diderrichii

Celine Blanc-Jolivet (1) , Malte Mader (1) , Henri-Noël Bouda (1) , Erwan Guichoux (2) , Germain Yene , Emmanuel Opuni-Frimpong (3, 4) , Bernd Degen (1)

1 Thünen Institute of Forest Genetics
2 BioGeCo - Biodiversité, Gènes & Communautés
3 UENR - University of Energy and Natural Resources
4 Forestry Research Institute of Ghana

The vulnerable African timber species Nauclea diderrichii is threatened because of its overexploitation and illegal trade. We sampled individuals across Ghana, Nigeria, Cameroon, Congo Brazzaville, Gabon and Democratic Republic of Congo and performed restriction associated DNA sequencing and low coverage MiSeq genome sequencing to discover SNP and INDEL loci. After MassARRAY genotyping of 95 individuals covering the sampled distribution range at 205 loci, a set of 88 loci were selected according to their differentiation among geographical regions. It is the first time that molecular markers are described for N. diderrichii and it should encourage genetic studies in this valuable species.

Mots clés: Nauclea diderrichii; Bilinga; single nucleotide polymorphism; MassARRAY

Domaines: Sciences du Vivant [q-bio]

Celine Blanc-Jolivet, Malte Mader, Henri-Noël Bouda, Erwan Guichoux, Germain Yene, et al.. Development of SNP markers for the African timber species Nauclea diderrichii. Conservation Genetics Resources, 2020, 12 (3), pp.357-359. ⟨10.1007/s12686-019-01115-w⟩. ⟨hal-02620082⟩