Development of nuclear SNP markers for genetic tracking of Iroko, Milicia excelsa and Milicia regia

C. Blanc-Jolivet (1) , B. Kersten (1) , K. Daïnou (2, 3) , O. Hardy (4) , Erwan Guichoux (5) , Adline Delcamp (5) , B. Degen (1)

1 Johann Heinrich von Thünen Institute
2 Nature+
3 Université Nationale d’Agriculture de Porto Novo
4 Faculté des Sciences, Evolutionary Biology and Ecology
5 BioGeCo - Biodiversité, Gènes & Communautés

Restriction associated DNA sequencing was conducted on two genetically independent individuals of Iroko, Milicia excelsa, for the discovery of nuclear SNPs. Ninety-four samples, well-distributed over the natural range and including timber DNA, were screened at 138 loci on a MassARRAY iPLEX system. Amplification success was high and 77 loci were selected to design a set of markers for genetic timber tracking purposes.

Mots clés: Single nucleotide polymorphism; milicia; RAD sequencing; mass ARRAY; timber tracking; timber

Mots clés: bois; marqueur génétique; polymorphisme des nucléotides simples

Domaines: Sciences du Vivant [q-bio]

C. Blanc-Jolivet, B. Kersten, K. Daïnou, O. Hardy, Erwan Guichoux, et al.. Development of nuclear SNP markers for genetic tracking of Iroko, Milicia excelsa and Milicia regia. Conservation Genetics Resources, 2017, online fisrt (4), pp.1-3. ⟨10.1007/s12686-017-0716-2⟩. ⟨hal-01607190⟩