Zobellia alginiliquefaciens sp. nov., a novel member of the flavobacteria isolated from the epibiota of the brown alga Ericaria zosteroides (C. Agardh) Molinari & Guiry 2020

Tristan Barbeyron (1) , Nolwen Le Duff (1) , Eric Duchaud (2) , François Thomas (1)

1 LBI2M - Laboratoire de Biologie Intégrative des Modèles Marins
2 VIM (UR 0892) - Virologie et Immunologie Moléculaires

Strain LLG6346-3.1 T , isolated from the thallus of the brown alga Ericaria zosteroides collected from the Mediterranean Sea near Bastia in Corsica, France, was characterised using a polyphasic method. Cells were Gram-stain-negative, strictly aerobic, non-flagellated, motile by gliding, rod-shaped and grew optimally at 30–33 °C, at pH 8–8.5 and with 4–5 % NaCl. LLG6346-3.1 T used the seaweed polysaccharide alginic acid as a sole carbon source which was vigorously liquefied. The results of phylogenetic analyses indicated that the bacterium is affiliated to the genus Zobellia (family Flavobacteriaceae , class Flavobacteriia ). LLG6346-3.1 T exhibited 16S rRNA gene sequence similarity values of 98.6 and 98.3 % to the type strains of Zobellia russellii and Zobellia roscoffensis , respectively, and of 97.4–98.5 % to members of other species of the genus Zobellia . The DNA G+C content of LLG6346-3.1 T was determined to be 38.3 mol%. Digital DNA–DNA hybridisation predictions by the average nucleotide identity (ANI) and genome to genome distance calculator (GGDC) methods between LLG6346-3.1 T and other members of the genus Zobellia showed values of 76–88 % and below 37 %, respectively. The results of phenotypic, phylogenetic and genomic analyses indicate that LLG6346-3.1 T is distinct from species of the genus Zobellia with validly published names and that it represents a novel species of the genus Zobellia , for which the name Zobellia alginiliquefaciens sp. nov. is proposed. The type strain is LLG6346-3.1 T (= RCC7657 T = LMG 32918 T ).

Mots clés: Zobellia alginiliquefaciens Flavobacteriaceae Alginate lyase Abbreviations: ANI; average nucleotide identity dDDH; digital DNA-DNA hybridization; Zobellia alginiliquefaciens; Flavobacteriaceae; Alginate lyase; Abbreviations: ANI; average nucleotide identity; dDDH

Domaines: Sciences du Vivant [q-bio]

Tristan Barbeyron, Nolwen Le Duff, Eric Duchaud, François Thomas. Zobellia alginiliquefaciens sp. nov., a novel member of the flavobacteria isolated from the epibiota of the brown alga Ericaria zosteroides (C. Agardh) Molinari & Guiry 2020. International Journal of Systematic and Evolutionary Microbiology, 2023, 73 (6), pp.005924. ⟨10.1099/ijsem.0.005924⟩. ⟨hal-04199452⟩