metaPR 2 : A database of eukaryotic 18S rRNA metabarcodes with an emphasis on protists

Daniel Vaulot (1) , Clarence Wei Hung Sim (2) , Denise Ong (2) , Bryan Teo (2) , Charlie Biwer (3) , Mahwash Jamy (3) , Adriana Lopes dos Santos (2)

1 ADMM - Adaptation et diversité en milieu marin
2 Nanyang Technological University [Singapour]
3 Uppsala Universitet [Uppsala]

Protists, that is, microbial eukaryotes that are not plants, animals or fungi (Archibald et al., 2017), are one of the most dominant life forms on earth, comprising up to 80% of the total eukaryotic diversity in the environment (de Vargas et al., 2015; Mahé et al., 2017; Massana et al., 2015). Protists play key ecological roles and are involved in primary productivity, nutrient cycling and carbon sequestration. It is thus crucial to assess protist diversity and the factors that determine community composition in order to predict how protists will respond to environmental change (Cavicchioli et al., 2019). While protists have historically been more difficult to study due to their small size, the explosion of metabarcoding studies over the past 10 years has greatly expanded our knowledge of these organisms (Burki et al., 2021; Santoferrara et al., 2020).

Mots clés: 18S rRNA; database; metabarcodes; PCR; protist; R; shiny

Domaines: Systématique, phylogénie et taxonomie; Bio-Informatique, Biologie Systémique [q-bio.QM]; Protistologie

Daniel Vaulot, Clarence Wei Hung Sim, Denise Ong, Bryan Teo, Charlie Biwer, et al.. metaPR 2 : A database of eukaryotic 18S rRNA metabarcodes with an emphasis on protists. Molecular Ecology Resources, In press, ⟨10.1111/1755-0998.13674⟩. ⟨hal-03765595⟩