Comparison of pulsed-field gel electrophoresis and whole-genome-sequencing-based typing confirms the accuracy of pulsed-field gel electrophoresis for the investigation of local Pseudomonas aeruginosa outbreaks

D. Martak (1, 2) , A. Meunier (1, 2) , M. Sauget (2, 1) , P. Cholley (1, 2) , M. Thouverez (1, 2) , X. Bertrand (2, 1) , Benoit Valot (2, 1) , D. Hocquet (2, 1)

1 CHRU Besançon - Centre Hospitalier Régional Universitaire de Besançon
2 LCE - Laboratoire Chrono-environnement (UMR 6249)

To determine whether pulsed-field gel electrophoresis (PFGE) accurately recognizes isolates belonging to clusters defined by techniques based on whole-genome sequencing (WGS) using Pseudomonas aeruginosa as a model.

Mots clés: Bacterial typing; Outbreaks; Pseudomonas aeruginosa; Pulsed-field gel electrophoresis; Whole-genome sequencing; cgMLST

Domaines: Microbiologie et Parasitologie

D. Martak, A. Meunier, M. Sauget, P. Cholley, M. Thouverez, et al.. Comparison of pulsed-field gel electrophoresis and whole-genome-sequencing-based typing confirms the accuracy of pulsed-field gel electrophoresis for the investigation of local Pseudomonas aeruginosa outbreaks. Journal of Hospital Infection, 2020, 105 (4), pp.643-647. ⟨10.1016/j.jhin.2020.06.013⟩. ⟨hal-03044091⟩