Comparison of double-locus sequence typing (DLST) and multilocus sequence typing (MLST) for the investigation of Pseudomonas aeruginosa populations.

Pascal Cholley (1, 2) , Milos Stojanov (3) , Didier Hocquet (1, 2) , Michelle Thouverez (1, 2) , Xavier Bertrand (1, 2) , Dominique S Blanc (4)

1 LCE - Laboratoire Chrono-environnement (UMR 6249)
2 CHRU Besançon - Centre Hospitalier Régional Universitaire de Besançon
3 Division of Molecular Genome Analysis
4 Service of Hospital Preventive Medicine

Reliable molecular typing methods are necessary to investigate the epidemiology of bacterial pathogens. Reference methods such as multilocus sequence typing (MLST) and pulsed-field gel electrophoresis (PFGE) are costly and time consuming. Here, we compared our newly developed double-locus sequence typing (DLST) method for Pseudomonas aeruginosa to MLST and PFGE on a collection of 281 isolates. DLST was as discriminatory as MLST and was able to recognize "high-risk" epidemic clones. Both methods were highly congruent. Not surprisingly, a higher discriminatory power was observed with PFGE. In conclusion, being a simple method (single-strand sequencing of only 2 loci), DLST is valuable as a first-line typing tool for epidemiological investigations of P. aeruginosa. Coupled to a more discriminant method like PFGE or whole genome sequencing, it might represent an efficient typing strategy to investigate or prevent outbreaks.

Domaines: Microbiologie et Parasitologie

Pascal Cholley, Milos Stojanov, Didier Hocquet, Michelle Thouverez, Xavier Bertrand, et al.. Comparison of double-locus sequence typing (DLST) and multilocus sequence typing (MLST) for the investigation of Pseudomonas aeruginosa populations.. Diagnostic Microbiology and Infectious Disease, 2015, 82 (4), pp.274-7. ⟨hal-01306065⟩