Rapid, sensitive and specific detection of OXA-48-like-producing Enterobacteriaceae by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry.

Marlène Sauget (1, 2) , Nadège Cabrolier (3) , Marion Manzoni , Xavier Bertrand (1, 2) , Didier Hocquet (1, 2)

1 CHRU Besançon - Centre Hospitalier Régional Universitaire de Besançon
2 LCE - Laboratoire Chrono-environnement (UMR 6249)
3 Service de Bactériologie [CHU Clermont-Ferrand]

: The emergence of Enterobacteriaceae producing the class D carbapenemase OXA-48 has become a public health concern, particularly in southern Europe and in the Mediterranean area. The existing MALDI-TOF MS assays that detect carbapenemase activity have disregarded the Enterobacteriaceae producing OXA-48-like enzymes. Here, we assessed the ability of an optimized MALDI-TOF MS imipenem hydrolysis assay to detect OXA-48-like-producing Enterobacteriaceae. We tested 372 non duplicate isolates of Enterobacteriaceae from 9 species, among which 92 harbored a blaOXA-48-like gene and 180 were non-susceptible to ertapenem. The vast majority (98.9%) of the OXA-48-like producers were detected and 97.8% of the ertapenem non-susceptible isolates were correctly classified. From the agar plate to the final result, the turnaround time of the test was 90min. This quick, easy and accurate MALDI-TOF MS test can expedite the implementation of hygiene measures to prevent dissemination of OXA-48-like-producing Enterobacteriaceae and limit outbreaks.

Domaines: Microbiologie et Parasitologie

Marlène Sauget, Nadège Cabrolier, Marion Manzoni, Xavier Bertrand, Didier Hocquet. Rapid, sensitive and specific detection of OXA-48-like-producing Enterobacteriaceae by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry.. Journal of Microbiological Methods, 2014, 105C, pp.88-91. ⟨10.1016/j.mimet.2014.07.004⟩. ⟨hal-01061607⟩