Multi-kernel deconvolution applied to confocal fluorescence microscopy with engineered point spread function

Bertrand Simon (1) , Olivier Haeberlé (1)

1 MIPS - Modélisation, Intelligence, Processus et Système

Fluorescence microscopy is a powerful technique in biology, because of the immense variety of markers now available. Compared to other methods, its resolution is however limited. In wide-field microscopy, the technique of structured illumination permits to improve the lateral resolution by a factor of two, even surpassing confocal microscopy, which permits a theoretical gain of about 40%. We propose an alternate technique, combining laterally interfering focused beams, which should permit the same gain of resolution in a confocal microscope. Furthermore, this technique, combined with multiple acquisition and multikernel deconvolution, permits a better object reconstruction than classical monokernel deconvolution using a regular excitation point spread function.

Mots clés: Point spread function engineering; multi-kernel deconvolution; confocal fluorescence microscopy

Domaines: Traitement du signal et de l'image [eess.SP]; Traitement du signal et de l'image [eess.SP]

Bertrand Simon, Olivier Haeberlé. Multi-kernel deconvolution applied to confocal fluorescence microscopy with engineered point spread function. Journal of the European Optical Society : Rapid publications, 2006, 1, pp.06028-1 - 06028-9. ⟨10.2971/jeos.2006.06028⟩. ⟨hal-00915873⟩