Non-radioactive in-situ hybridisation to honeybee embryos and ovaries

Peter Osborne , Peter K. Dearden

To study the function and expression of genes identified in the honeybee genome sequence, techniques for assaying gene expression need to be developed. We present a robust method for preparing honeybee embryos and ovaries for in-situ hybridisation. This protocol should prove useful for researchers wishing to use the genome sequence to study honeybee biology.

Mots clés: Apis mellifera; embryo; ovary; gene expression; in-situ hybridisation

Domaines: Zoologie des invertébrés; Biodiversité; Ecologie, Environnement; Science des productions animales

Peter Osborne, Peter K. Dearden. Non-radioactive in-situ hybridisation to honeybee embryos and ovaries. Apidologie, 2005, 36 (1), pp.113-118. ⟨hal-00892122⟩