Nanocomposite Carbon-PDMS thick electrodes for electrokinetic manipulation during cell fusion

Mathieu Brun (1) , Anne-Laure Deman (1) , Jean-François Chateaux (1) , Marie Frénéa-Robin (2) , Naoufel Haddour (2) , Rosaria Ferrigno (1)

1 INL - LOCI - INL - Lab-On-Chip et Instrumentation
2 AMPERE - Ampère

This paper reports on the integration and evaluation of robust, thick Carbon-PDMS nanocomposite electrodes (CPDMS), integrated in PDMS microfluidic systems to carry out electric field induced fusion of biological cells. Such material preserves PDMS processing properties and sustains a large range of electric field intensities and frequencies without any carbon release. Polystyrene particles, yeasts and HEK-293 cells were manipulated in devices consisting of C-PDMS walls bounded to a glass plate. Different collective behaviors were observed such as alignment in chains parallel to the electric field and in circulating bands tilted with respect to the electric field direction. An AC electrofusion protocol was also tested.

Mots clés: Microfluidics; Electrokinetics; Carbon-Polydimethylsiloxane Nanocomposite; Cell Electrofusion

Domaines: Energie électrique

Mathieu Brun, Anne-Laure Deman, Jean-François Chateaux, Marie Frénéa-Robin, Naoufel Haddour, et al.. Nanocomposite Carbon-PDMS thick electrodes for electrokinetic manipulation during cell fusion. MicroTAS, Oct 2010, Groningen, Netherlands. pp.911-913. ⟨hal-00734010⟩