Synthesis of Homo- and Heterofunctionalized Glycoclusters and Binding to Pseudomonas aeruginosa Lectins PA-IL and PA-IIL

Béatrice Gerland (1) , Alice Goudot (2) , Glawdys Pourceau (1) , Albert Meyer (1) , Sébastien Vidal (3) , Eliane Souteyrand (2) , Jean-Jacques Vasseur (1) , Yann Chevolot (2) , François Morvan (1)

1 IBMM - Institut des Biomolécules Max Mousseron [Pôle Chimie Balard]
2 INL - C&N - INL - Chimie et Nanobiotechnologies
3 ICBMS - Institut de Chimie et Biochimie Moléculaires et Supramoléculaires

Homo- and heterofunctionalized glycoclusters with galactose and/or fucose residues targeting both PA-IL and PA-IIL lectins of Pseudomonas aeruginosa were synthesized using "Click" chemistry and DNA chemistry. Their binding to lectins (separately or in a mixture) was studied using a DNA Directed Immobilization carbohydrate microarray. Homoglycoclusters bind selectively to their lectin while the heteroglycocluster binds simultaneously both lectins with a slight lower affinity.

Domaines: Chimie organique

Béatrice Gerland, Alice Goudot, Glawdys Pourceau, Albert Meyer, Sébastien Vidal, et al.. Synthesis of Homo- and Heterofunctionalized Glycoclusters and Binding to Pseudomonas aeruginosa Lectins PA-IL and PA-IIL. Journal of Organic Chemistry, 2012, 77, pp.7620-7626. ⟨10.1021/jo300826u⟩. ⟨hal-00729088⟩