Evaluation of LC - high-resolution FT - Orbitrap MS for the quantification of selected mycotoxins and the simultaneous screening of fungal metabolites in food
Résumé
This study presents a liquid chromatography - high-resolution mass spectrometry based method for the quantification of 20 selected mycotoxins and the simultaneous screening for 200 fungal metabolites in food. For regulated mycotoxins, such as aflatoxins, fumonisins, ochratoxin A, zearalenone and trichothecenes, evaluation of the method performance characteristics precision, trueness, limit of detection and matrix effects have been exemplified for the matrix maize. In case of the limit of detection, an alternative evaluation approach for high resolution FT-Orbitrap data is proposed. Measurements of the signal to noise ratios obtained from "full profile mode" data led to detection limits between 8 and 160 ng g-1. Eight naturally contaminated, wheat- and maize-based matrix test materials, originating from interlaboratory comparison studies were used to confirm trueness of the method for deoxynivalenol, zearalenone, fumonisin B1 and B2, HT-2 and T-2 toxin. In addition to accurate quantification of the most relevant mycotoxins, the obtained full scan chromatograms were used to investigate the potential of the FT-Orbitrap to screen simultaneously for a large number of fungal metabolites. First, a list of 200 metabolites, potentially being present in food samples has been established. Next, specific detection and identification criteria were defined, which were based on accurate mass, peak intensity and isotopologue ratio. Application of these criteria to the suspected metabolites from the list resulted in the putative identification of 13 fungal metabolites in addition to the target toxins.
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