Divergent cytokine responses of macrophages to subsp. strains of Type II and III in a standardized model

Erika Borrmann (1) , Petra Möbius (1) , Roland Diller (1) , Heike Köhler (1)

1 Institute of Molecular Pathogenesis

Based on epidemiological and clinical observations, different strains of subsp (MAP) are suspected to significantly differ in their virulence for ruminants. In the pathogenesis of paratuberculosis, macrophages represent the principal target cell for MAP. In order to judge the ability of different MAP-genotypes to modulate macrophage responses, the cytokine responses of the monocyte cell line THP-1 were studied after challenge with three different MAP strains under standardized conditions. The bovine field isolate J1961 (major Type II) and the ovine field isolate JIII-86 (Type III) were compared with the laboratory adapted reference strain ATCC 19698 (Type II). Strains were shown by three different typing methods (IS900-RFLP-, MIRU-VNTR-, and SSR-analysis) to substantially differ in several genotypic features. Macrophage function was assessed by quantifying mRNA of the cytokines TNF-α, IL-1ß, and IL-10 by quantitative RT-PCR. Secreted TNF-α protein was measured by a cytotoxicity test, IL-1ß and IL-10 using ELISA tests.

Mots clés: subsp; subsp.; MAP-genotypes; cytokines; THP-1; quantitative real-time PCR

Domaines: Médecine vétérinaire et santé animale

Erika Borrmann, Petra Möbius, Roland Diller, Heike Köhler. Divergent cytokine responses of macrophages to subsp. strains of Type II and III in a standardized model. Veterinary Microbiology, 2011, 152 (1-2), pp.101. ⟨10.1016/j.vetmic.2011.04.002⟩. ⟨hal-00719075⟩