Genetic variability of strains assessed by PCR-RFLP analysis of polymorphic membrane protein-encoding genes
Résumé
This study used PCR-RFLP to investigate the genetic variability of -encoding genes from fifty-two () strains originating from abortion cases from various geographical regions and host species. Six primer pairs were used to PCR-amplify DNA fragments encoding eighteen pmps. PCR products were digested using four restriction endonucleases and Bayesian methodologies were used to compare RFLP profiles and assign strains to a RFLP genotype. Strains could be assigned to 2 genotypes in the region encoding , 3 genotypes in the regions encoding , and , 4 genotypes in the region encoding and 5 genotypes in the region encoding -. In all regions, the majority of strains (88.4%-96.1%) had the same genotype as the reference strain S26/3. No correlation could be made between genotype, host species or geographical origin except for the two variant Greek strains, LLG and POS, which formed a discrete genotype in all -encoding regions except . Relative rates of evolution calculated for each -encoding gene locus suggest that differing selective pressures and functional constraints may exist on polymorphic membrane proteins. These findings suggest that although intraspecies heterogeneity of pmp-encoding genes in is low, the sequence heterogeneity should be an important consideration when using pmps as the basis for novel diagnostics or vaccine development.
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