Occurrence and virulence patterns of O26, O103, O111 and O145 in slaughter cattle
Résumé
The study attempted to investigate the occurrence of non-O157 serogroups O26, O103, O111 and O145 in cattle at slaughter and to determine the virulence potential of these isolates. A total of 399 fecal samples were analyzed by selective plating and isolates were characterized by polymerase chain reaction (PCR) for the genes and EHEC . Immunomagnetic separation (IMS) is required to increase the efficiency of the isolation procedure. O26, O103, O111 and O145 were recovered from 24 (6%) fecal samples. O26 and O103 seemed to be more abundant in slaughter cattle than O111 and O145. Sixteen out of the 24 isolates harbored genes. All -positive isolates harbored one or more additional virulence factors. Six out of the 8 -negative isolates harbored and/or EHEC , whereas 2 strains harbored none of the tested virulence genes.
Origine : Fichiers produits par l'(les) auteur(s)