Immobilized enzymes affect biofilm formation

Ana L. Cordeiro (1) , Catharina Hippius (2) , Carsten Werner (1)

1 IPF - Leibniz-Institut für Polymerforschung Dresden e.V.
2 BASF SE

The effect of the activity of immobilized enzymes on the initial attachment of pathogenic bacteria commonly associated with nosocomial infections ( and ) was investigated. The proteolytic enzymes, subtilisin A and the glycoside hydrolase cellulose, were covalently attached onto poly(ethylene--maleic) anhydride copolymer films. A comparison between active and heat-inactivated surfaces showed that while the activity of immobilized cellulase reduced the attachment of by 67%, it had no effect on the attachment of . Immobilized subtilisin A had opposite effects: the active enzyme had no effect on the attachment of but reduced the attachment of by 44%. The results suggest that different biomolecules are involved in the initial steps of attachment of different bacteria, and that the development of broad-spectrum antifouling enzymatic coatings will need to involve the co-immobilization of enzymes.

Mots clés: Bioactive surfaces; Biofilm; Cellulase; Subtilisin

Domaines: Biotechnologie; Biotechnologies

Ana L. Cordeiro, Catharina Hippius, Carsten Werner. Immobilized enzymes affect biofilm formation. Biotechnology Letters, 2011, 33 (9), pp.1897-1904. ⟨10.1007/s10529-011-0643-3⟩. ⟨hal-00701875⟩