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| HAL : hal-00691511, version 1 |
| PubMed : 21739555 |
| DOI : 10.1002/cbic.201100128 |
| Fiche détaillée |  Récupérer au format |
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| ChemBioChem 12, 13 (2011) 2071-80 |
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| Measurement of enzymatic activity and specificity of human and avian influenza neuraminidases from whole virus by glycoarray and MALDI-TOF mass spectrometry. |
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| Gwladys Pourceau 1Yann Chevolot 2 |
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| (05/09/2011) |
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| Influenza neuraminidases hydrolyze the ketosidic linkage between N-acetylneuraminic acid and its adjacent galactose residue in sialosides. This enzyme is a tetrameric protein that plays a critical role in the release of progeny virions. Several methods have been described for the determination of neuraminidase activity, usually based on colorimetric, fluorescent, or chemiluminescent detection. However, only a few of these tests allow discrimination of the sialyl-linkage specificity (i.e., α2-3- versus α2-6-linked sialyllactosides) of the neuraminidase. Herein we report a glycoarray-based assay and a MALDI-TOF study for assessing the activity and specificity of two influenza neuraminidases on whole viruses. The human A(H3N2) and avian A(H5N2) neuraminidase activities were investigated. The results from both approaches demonstrated that α2-3 sialyllactoside was a better substrate than α2-6 sialyllactoside for both viruses and that H5N2 virus had a lower hydrolytic activity than H3N2. |
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| 1 : | Institut des Biomolécules Max Mousseron (IBMM) |
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CNRS : UMR5247 – Université Montpellier I – Université Montpellier II - Sciences et techniques |
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| 2 : | Institut des nanotechnologies de Lyon - Site d'Ecully (INL) |
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CNRS : UMR5270 – Université Claude Bernard - Lyon I – Institut National des Sciences Appliquées (INSA) - Lyon – Ecole Centrale de Lyon |
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| 3 : | Virologie et Pathologie Humaine (VirPath) |
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CNRS : FRE3011 – Université Claude Bernard - Lyon I – École Normale Supérieure - Lyon |
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| 4 : | Institut de Chimie et Biochimie Moléculaires et Supramoléculaires (ICBMS) |
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CNRS : UMR5246 – Université Claude Bernard - Lyon I – Institut National des Sciences Appliquées (INSA) - Lyon – École Supérieure Chimie Physique Électronique de Lyon |
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| 5 : | University of California |
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University of California |
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| Domaine | : | Chimie/Chimie organique |
| hal-00691511, version 1 | |
| http://hal.archives-ouvertes.fr/hal-00691511 | |
| oai:hal.archives-ouvertes.fr:hal-00691511 | |
| Contributeur : Frédérique Depierre | |
| Soumis le : Jeudi 26 Avril 2012, 14:13:20 | |
| Dernière modification le : Jeudi 26 Avril 2012, 14:13:20 | |
