Glycoarray by DNA-directed immobilization.

François Morvan (1) , Yann Chevolot (2) , Jing Zhang (3) , Albert Meyer (1) , Sébastien Vidal (4) , Jean-Pierre Praly (4) , Jean-Jacques Vasseur (1) , Eliane Souteyrand (2)

1 IBMM - Institut des Biomolécules Max Mousseron [Pôle Chimie Balard]
2 INL - C&N - INL - Chimie et Nanobiotechnologies
3 LMVI - Key Laboratory of Molecular Virology & Immunology
4 ICBMS - Institut de Chimie et Biochimie Moléculaires et Supramoléculaires

Glycoarrays have become a powerful platform to investigate the interactions of many biological events involving carbohydrates. The carbohydrates immobilization on the surface of the substrates is a key step of glycoarray fabrication. Plenty of strategies have been applied to the immobilization process. Herein a protocol for the synthesis of oligonucleotide glycomimetic conjugates is proposed. The resulting molecules are immobilized by hybridization on a DNA microarray (DNA-directed immobilization; DDI). DDI has been proved to be a very efficient and site-selective. This protocol provides detailed procedures for the preparation of fluorescent oligonucleotide trigalactosylmimetic conjugates and for the preparation of carbohydrate microarrays by DDI on glass slides.

Domaines: Chimie organique

François Morvan, Yann Chevolot, Jing Zhang, Albert Meyer, Sébastien Vidal, et al.. Glycoarray by DNA-directed immobilization.. Methods in Molecular Biology, 2012, 808, pp.195-219. ⟨10.1007/978-1-61779-373-8_14⟩. ⟨hal-00691496⟩