Two-phase temporary immersion system for genetic transformation of sage ( Mill.)

Andrey Marchev (1) , Vasil Georgiev (1) , Ivan Ivanov (1) , Ilian Badjakov (2) , Atanas Pavlov (1)

1 Institut Stephan Angeloff
2 AgroBioInstitute

Hairy root cultures of were initiated by transformation with . To prevent necrosis in the explants and to protect young hairy roots, Amberlite XAD-4 resin, in combination with a temporary immersion cultivation system, was applied. HPLC analyzes showed that the resin adsorbed more than 93% of the released phenolic acids and 100% of the released flavonoids. The decreased content of the released phenolics significantly reduced their destructive effects on the plant tissues, prevented, and speeded up the appearance of hairy roots.

Mots clés: Amberlite XAD4; Necrosis; Phenolics; Sage; Mill.; Mill

Domaines: Biotechnologie; Biotechnologies

Andrey Marchev, Vasil Georgiev, Ivan Ivanov, Ilian Badjakov, Atanas Pavlov. Two-phase temporary immersion system for genetic transformation of sage ( Mill.). Biotechnology Letters, 2011, 33 (9), pp.1873-1878. ⟨10.1007/s10529-011-0625-5⟩. ⟨hal-00690284⟩