pGOODs: new plasmids for the co-expression of proteins in

Emanuele Conte (1) , Gianpiero Landolfi (1) , Gabriele Vincelli (1) , Alessandra Stefan (1, 2) , Alejandro Hochkoeppler (1, 2)

1 Department of Industrial Chemistry
2 CSGI, Department of Chemistry

Two systems for the co-expression of proteins in were designed and constructed. The first system relies on the new vector, pGOOD, which is compatible with ColE1-type plasmids and sustains efficient co-expression of soluble protein complexes. The second system is based on the pGOOD1 vector (a derivative of pGOOD), useful for the production of toxic proteins, whose synthesis can be regulated by the co-expressed LacI repressor.

Mots clés: ColE1 origin; Compatible plasmid; Expression vector; p15A origin

Domaines: Biotechnologie; Biotechnologies

Emanuele Conte, Gianpiero Landolfi, Gabriele Vincelli, Alessandra Stefan, Alejandro Hochkoeppler. pGOODs: new plasmids for the co-expression of proteins in. Biotechnology Letters, 2011, 33 (9), pp.1815-1821. ⟨10.1007/s10529-011-0624-6⟩. ⟨hal-00690283⟩