Possible role of azole and echinocandin lock solutions in the control of biofilms associated with silicone

Estelle Cateau (1) , Jean-Marc Berjeaud (1) , Christine Imbert (1)

1 LCME - Laboratoire de chimie et microbiologie de l'eau [Poitiers]

Until now, management of candidiasis related to implanted devices has remained problematic. The aim of this study was to investigate antifungal lock strategies against and biofilms in vitro. Three antifungal agents were used against eight and six . clinical strains isolated from infected catheters. Caspofungin and micafungin, both echinocandins, as well as the azole posaconazole were tested. An in vitro model of biofilm on 100% silicone catheters was used. Efficacy of the antifungal lock was tested against biofilms aged 12h and 5 days following exposure to caspofungin (5mg/L and 25mg/L), micafungin (5mg/L and 15mg/L) and posaconazole (10mg/L) for 12h. Persistence of antibiofilm activity was investigated 1-3 days following drug elimination. Antifungal lock was considered effective in the event of a significant decrease ( < 0.001) in the metabolic activity of the biofilm yeast. The results showed that micafungin had significant inhibitory effectiveness against young and mature and biofilms. Moreover, this activity appeared to persist for up to 3 days. Caspofungin displayed similar activity against all biofilms, but the activity was less persistent for biofilms. Posaconazole was less effective against C. biofilms, but its activity was sustained. Echinocandin lock therapy could significantly enhance the management of candidiasis in patients with indwelling catheters by combating biofilms and enabling device maintenance in situ.

Mots clés: Candida; Biofilm; Lock solution; Echinocandin; Posaconazole

Domaines: Microbiologie et Parasitologie

Estelle Cateau, Jean-Marc Berjeaud, Christine Imbert. Possible role of azole and echinocandin lock solutions in the control of biofilms associated with silicone. International Journal of Antimicrobial Agents, 2011, 37 (4), pp.380. ⟨10.1016/j.ijantimicag.2010.12.016⟩. ⟨hal-00679595⟩