Anti-inflammatory actions of a taurine analogue; ethane β-sultam; in phagocytic cells; in vivo and in vitro

Roberta J Ward (1, 2) , Frederic Lallemand , Philippe de Witte , Robert R Crichton (3) , Jacques Piette (4) , Keith Tipton (5) , Karl Hemmings (6) , Arnaud Pitard (6) , Mike Page (6) , Laura Della Corte (1) , Deanna Taylor (2) , David Dexter (2)

1 UniFI - Università degli Studi di Firenze = University of Florence = Université de Florence
2 Imperial College London
3 UCL - Université Catholique de Louvain = Catholic University of Louvain
4 Université de Liège
5 Trinity College Dublin
6 University of Huddersfield

The ability of a taurine prodrug, ethane β-sultam, to reduce cellular inflammation has been investigated, , in primary cultures of alveolar macrophages and an immortilised N9 microglial cell line and in an animal model of inflammation and control rats. Ethane β-sultam showed enhanced ability to reduce the inflammatory response in alveolar macrophages, as assayed by the lipopolysaccharide-stimulated-nitric oxide release, (LPS stimulated-NO), in comparison to taurine both (10nM, 50nM) and in vivo (0.15mmol/kg/day by gavage). In addition, ethane β-sultam, (50, 100 and 1000nM) significantly reduced LPS-stimulated glutamate release from N9 microglial cells to a greater extent than taurine. The anti-inflammatory response of taurine was shown to be mediated via stabilisation of IkBα. The use of a taurine prodrug as therapeutic agents, for the treatment of neurological conditions, such as Parkinson's and Alzheimer's disease and alcoholic brain damage, where activated phagocytic cells contribute to the pathogenesis, may be of great potential.

Mots clés: taurine: anti-inflammatory action: ethane-β sultam: nitric oxide: glutamate

Domaines: Pharmacologie

Roberta J Ward, Frederic Lallemand, Philippe de Witte, Robert R Crichton, Jacques Piette, et al.. Anti-inflammatory actions of a taurine analogue; ethane β-sultam; in phagocytic cells; in vivo and in vitro. Biochemical Pharmacology, 2011, 81 (6), pp.743. ⟨10.1016/j.bcp.2010.12.030⟩. ⟨hal-00672299⟩