Analyses of human papillomavirus genotypes and viral loads in anogenital warts.

Siolian Liewang Rita Ball (1) , David Winder (1) , Katie Vaughan (1) , Nashat Hanna (2) , Jonathan Andre Michel Salomon Levy (3) , Jane Sterling (1) , Margaret Stanley (4) , Peter Goon (1)

1 Division of Virology, Department of Pathology
2 GU/HIV Medicine
3 UPD7 - Université Paris Diderot - Paris 7
4 Pathology

Condylomata acuminata (genital warts) are the most common sexually transmitted viral diseases. These lesions are caused by infection with mucosal human papillomaviruses (HPVs). However there is limited information on HPV strain distribution involved in the molecular pathogenesis of these lesions. To address this the strain prevalence and the frequency of multiple HPV infections in wart tissue were examined in samples from 31 patients attending a wart clinic. These lesions were bisected and subjected to parallel DNA and mRNA extractions. HPV-type prevalence and incidence of multiple infections were determined by the Roche Linear Array assay. qPCR compared HPV6, 11 16 and 18 viral loads and RT-qPCR measured HPV 6 and 11 E6 genomic expression levels. 71% of these samples were infected with multiple HPVs. Only 1 sample was negative for HPV6 or 11 DNA. 48% of samples were positive for a high risk (oncogenic) HPV. These results show that multiple infections in tissue are frequent and the subsequent analysis of HPV6 and 11 E6 DNA viral loads suggested that other HPVs could be causing lesions. Further analysis of HPV6/11 E6 mRNA levels showed that there was no discernable relationship between HPV6 E6 DNA viral load and relative HPV 6 or 11 E6 mRNA levels thereby questioning the relevance of viral load to lesion causality.

Mots clés: Medicine

Siolian Liewang Rita Ball, David Winder, Katie Vaughan, Nashat Hanna, Jonathan Andre Michel Salomon Levy, et al.. Analyses of human papillomavirus genotypes and viral loads in anogenital warts.. Journal of Medical Virology, 2011, 83 (8), pp.1345. ⟨10.1002/jmv.22111⟩. ⟨hal-00652144⟩