Production of human metabolites of the anti-cancer drug flutamide via biotransformation in species

Jessica Amadio (1) , Cormac D. Murphy (1)

1 School of Biomolecular and Biomedical Science

Fungi belonging to the genus have enzymes similar to those employed by mammals for the detoxification of xenobiotics, thus they are useful as models of mammalian drug metabolism, and as a source for drug metabolites. We report the transformation of the anti-cancer drug flutamide in sp. The most predominant phase I metabolites present in the plasma of humans, 2-hydroxyflutamide and 4-nitro-3-(trifluoromethyl)aniline, were also produced in cultures. Other phase I and phase II metabolites were also detected using a combination of HPLC, GC-MS and F-NMR.

Mots clés: Biotransformation; Cytochrome P450; Fluorine-19 NMR; Sulfotransferase

Jessica Amadio, Cormac D. Murphy. Production of human metabolites of the anti-cancer drug flutamide via biotransformation in species. Biotechnology Letters, 2010, 33 (2), pp.321-326. ⟨10.1007/s10529-010-0425-3⟩. ⟨hal-00630269⟩