Mutanase from sp. MP-1 produced inductively by fungal α-1,3-glucan and its potential for the degradation of mutan and biofilm

M. Pleszczyńska (1) , A. Wiater (1) , J. Szczodrak (1)

1 Department of Industrial Microbiology

is a source of α-1,3-glucan that can substitute for the commercially-unavailable streptococcal mutan used to induce microbial mutanases. The water-insoluble fraction of its fruiting bodies from 0.15 to 0.2% (w/v) induced mutanase activity in sp. MP-1 at 0.35 μ ml. The mutanase extensively hydrolyzed streptococcal mutan, giving 23% of saccharification, and 83% of solubilization of glucan after 6 h. It also degraded α-1,3-polymers of biofilms, formed in vitro by , even after only 3 min of contact.

Mots clés: α-1; 3-Glucan; Mutanase

M. Pleszczyńska, A. Wiater, J. Szczodrak. Mutanase from sp. MP-1 produced inductively by fungal α-1,3-glucan and its potential for the degradation of mutan and biofilm. Biotechnology Letters, 2010, 32 (11), pp.1699-1704. ⟨10.1007/s10529-010-0346-1⟩. ⟨hal-00607699⟩