Analysis of the functional consequences of lethal mutations in mitochondrial translational elongation factors

Kenta Akama (1) , Brooke E. Christian (2) , Christie N. Jones (2) , Takuya Ueda (1) , Nono Takeuchi (1) , Linda L. Spremulli (2)

1 Department of Medical Genome Sciences
2 Department of Chemistry

Mammalian mitochondria synthesize a set of thirteen proteins that are essential for energy generation via oxidative phosphorylation. The genes for all of the factors required for synthesis of the mitochondrially-encoded proteins are located in the nuclear genome. A number of disease-causing mutations have been identified in these genes. In this manuscript, we have elucidated the mechanisms of translational failure for two disease states characterized by lethal mutations in mitochondrial elongation factor Ts (EF-Ts) and elongation factor Tu (EF-Tu).

Mots clés: EF-Ts; aa-tRNA; EF-G1; EF-Tu; Translation; Elongation; Mitochondria; Disease

Kenta Akama, Brooke E. Christian, Christie N. Jones, Takuya Ueda, Nono Takeuchi, et al.. Analysis of the functional consequences of lethal mutations in mitochondrial translational elongation factors. Biochimica et Biophysica Acta - Molecular Basis of Disease, 2010, 1802 (7-8), pp.692. ⟨10.1016/j.bbadis.2010.04.003⟩. ⟨hal-00600123⟩