Oral rinse MMP-8 point-of-care test identifies patients with strong periodontal inflammatory burden

Jussi Leppilahti (1) , Minna-Maija Ahonen (1) , Marcela Hernández (2) , Sarvan Munjal (3) , Lutz Netuschil (3) , Veli-Jukka Uitto (1, 4) , Timo Sorsa (1, 5) , Päivi Mäntylä (1)

1 Institute of Dentistry
2 Periodontal biology
3 dentognostics GmbH
4 Department of Oral and Maxillofacial Diseases
5 Department of Oral and Maxillofacial Diseases

Objective: The aim of the study was to determine whether oral rinse MMP-8 levels, measured by three different methods, TIMP-1 levels and elastase activity differentiate subjects with different periodontal condition; and second, to find out if MMP-8 levels were comparable among the methods used. Methods: MMP-8 levels were analyzed with an immunofluorometric method (IFMA), dentoAnalyzer and commercial ELISA. Also TIMP-1 levels and elastase activity were measured. For statistical analysis 214 study subjects were categorized into four groups, specified by the presence and number of moderate (4-5 mm) and deep (≥ 6 mm) periodontal pockets, and bleeding on probing percentage. Results: MMP-8 levels especially measured by dentoAnalyzer and adjusted to the number of teeth per subject differentiated the study group with strong periodontal inflammatory burden from groups with lower levels. This was also verified with receiver operating characteristic (ROC) analysis. Elastase activity associated with higher IFMA and dentoAnalyzer MMP-8 levels. IFMA MMP-8/TIMP and dentoAnalyzer MMP-8/TIMP-1 tended to be higher with the increasing level of periodontal inflammatory burden. TIMP-1 levels decreased with increasing age. Conclusions: Oral rinse MMP-8 together with TIMP-1 analysis may have potential in complementary periodontal diagnostics. dentoAnalyzer can be applied in quantitative oral rinse chair side biomarker diagnostics.

Mots clés: Medicine

Jussi Leppilahti, Minna-Maija Ahonen, Marcela Hernández, Sarvan Munjal, Lutz Netuschil, et al.. Oral rinse MMP-8 point-of-care test identifies patients with strong periodontal inflammatory burden. Oral Diseases, 2010, 17 (1), pp.115. ⟨10.1111/j.1601-0825.2010.01716.x⟩. ⟨hal-00599812⟩