Analytical strategy for the determination of non-steroidal anti-inflammatory drugs in plasma and improved analytical strategy for the determination of authorised and non-authorised non-steroidal anti-inflammatory drugs in milk by liquid chromatography tandem mass spectrometry

Geraldine Dowling (1) , Edward Malone (2) , Tom Harbison (2) , Sheila Martin (2)

1 The State Laboratory
2 The State Laboratory

A sensitive and selective method for the determination of 6 non-steroidal anti-inflammatory drugs in bovine plasma was developed. An improved method for the determination of authorised and non-authorised residues of 10 non-steroidal anti-inflammatory drugs in milk was developed. Analytes were separated and acquired by liquid chromatography coupled with electrospray ionisation tandem mass spectrometer (LC-ESI-MS/MS). Target compounds were acidified in plasma and plasma and milk samples were extracted with acetonitrile and both extracts were purified on an improved solid phase extraction procedure utilising Evolute TM ABN solid phase extraction cartridges. Accuracy of the methods in milk and plasma was between 73 and 109 %. The precision of the method for authorised and non-authorised NSAIDs in milk and plasma expressed as % RSD , for the within -laboratory reproducibility was less than 16 % and for authorised NSAIDs of meloxicam, flunixin and tolfenamic acid at their associated MRLs in milk was less than 10 % however for hydroxy flunixin was less than 25%. The methods were validated according to Commission Decision 2002/657/EC.

Mots clés: Life Sciences

Geraldine Dowling, Edward Malone, Tom Harbison, Sheila Martin. Analytical strategy for the determination of non-steroidal anti-inflammatory drugs in plasma and improved analytical strategy for the determination of authorised and non-authorised non-steroidal anti-inflammatory drugs in milk by liquid chromatography tandem mass spectrometry. Food Additives and Contaminants, 2010, 27 (07), pp.962-982. ⟨10.1080/19440041003706779⟩. ⟨hal-00598945⟩