Esterification of phenolic acids catalyzed by lipases immobilized in organogels

M. Zoumpanioti (1) , E. Merianou (1, 2) , T. Karandreas (1) , H. Stamatis (2) , A. Xenakis (1)

1 Institute of Biological Research and Biotechnology
2 Biological Applications and Technologies Department

Lipases from and B were immobilized in hydroxypropylmethyl cellulose organogels based on surfactant-free microemulsions consisting of n-hexane, 1-propanol and water. Both lipases kept their catalytic activity, catalyzing the esterification reactions of various phenolic acids including cinnamic acid derivatives. High reaction rates and yields (up to 94%) were obtained when lipase from was used. Kinetic studies have been performed and apparent kinetic constants were determined showing that ester synthesis catalyzed by immobilized lipases occurs via the Michaelis-Menten mechanism.

Mots clés: Biocatalysis; Antioxidants; Hydroxypropylmethyl cellulose

M. Zoumpanioti, E. Merianou, T. Karandreas, H. Stamatis, A. Xenakis. Esterification of phenolic acids catalyzed by lipases immobilized in organogels. Biotechnology Letters, 2010, 32 (10), pp.1457-1462. ⟨10.1007/s10529-010-0305-x⟩. ⟨hal-00594822⟩