The same synaptic vesicles drive active and spontaneous release

Benjamin Gerhard Wilhelm (1) , Teja Wolfgang Groemer (1) , Silvio O. Rizzoli (1)

1 STED Microscopy

Synaptic vesicles release neurotransmitter both actively (upon stimulation) and spontaneously (at rest). It has been long assumed that identical vesicles use both modes of release; however, recent evidence has challenged this view. Using several assays (FM dye imaging, pHluorin imaging and antibody-labeling of synaptotagmin), in neuromuscular preparations from Drosophila, frog and mouse as well as rat cultured neurons, we suggest that the same vesicles participate in active and spontaneous release.

Mots clés: Synaptic Transmission; Neurophysiology; Neuroimaging

Benjamin Gerhard Wilhelm, Teja Wolfgang Groemer, Silvio O. Rizzoli. The same synaptic vesicles drive active and spontaneous release. Nature Neuroscience, 2010, ⟨10.1038/nn.2690⟩. ⟨hal-00594791⟩