Characterisation of the CTX-M-15-encoding gene in Klebsiella pneumoniae strains from the Barcelona metropolitan area: plasmid diversity and chromosomal integration

Alicia Coelho (1, 2) , Juan José González-López (1, 2) , Elisenda Miró (3) , Carles Alonso-Tarrés (4) , Beatriz Mirelis (3, 2) , María Nieves Larrosa (1, 2) , Rosa María Bartolomé (1, 2) , Antonia Andreu (1, 2) , Ferran Navarro (3, 2) , James R. Johnson (5) , Guillem Prats (1, 2)

1 Vall d'Hebron University Hospital [Barcelona]
2 UAB - Universitat Autònoma de Barcelona = Autonomous University of Barcelona = Universidad Autónoma de Barcelona
3 Hospital de la Santa Creu i Sant Pau
4 Hospital General de l'Hospitalet
5 Veterans Affairs Medical Center and University of Minnesota

The localisation and genetic organisation of were studied in 37 CTX-M-15-producing isolates collected from 2005 to 2008 within the Barcelona metropolitan area. Polymerase chain reaction (PCR)-based replicon typing and Southern hybridisations were used to identify the location. The genetic environment was analysed by PCR mapping and sequencing, and transferability of was evaluated by conjugation and transformation assays. The majority of the 37 isolates carried in a plasmid location, frequently associated with the gene. Plasmids encoding carried three distinct replicons, i.e. IncFII, IncR and IncFIIk, the latter two not having been described previously in association with . Several of these plasmids were not self-transferable. Furthermore, in all isolates belonging to sequence type ST-1, was found integrated into the chromosome. In all the studied isolates, the mobile element IS was found upstream of , whereas IS was found inserted within IS in several isolates, in previously unreported positions. In conclusion, these findings indicate that among strains isolated in the Barcelona metropolitan area, is associated with diverse genetic elements, including the IncR and IncFIIk replicons, as reported for the first time here, and the chromosome.

Mots clés: bla; Replicon; Klebsiella pneumoniae

Alicia Coelho, Juan José González-López, Elisenda Miró, Carles Alonso-Tarrés, Beatriz Mirelis, et al.. Characterisation of the CTX-M-15-encoding gene in Klebsiella pneumoniae strains from the Barcelona metropolitan area: plasmid diversity and chromosomal integration. International Journal of Antimicrobial Agents, 2010, 36 (1), pp.73. ⟨10.1016/j.ijantimicag.2010.03.005⟩. ⟨hal-00594504⟩