Relaxant effect of brain natriuretic peptide in nonsensitized and passively sensitized isolated human bronchi

Maria Gabriella Matera , Luigino Calzetta , Vincenzo Parascandolo , Giacomo Curradi , Paola Rogliani , Mario Cazzola

Brain natriuretic peptide (BNP) relaxes guinea pig tracheal smooth muscle and is effective in preventing ovalbumin-induced bronchoconstriction and microvascular leakage in guinea pigs Nonetheless, published studies on BNP in human airways are still lacking in the literature. The aim of this study was to investigate the effect of BNP in isolated human bronchi. The relaxant effect of BNP (1nM to 10μM) was assessed in nonsensitized and in passively sensitized human bronchial airways pre-contracted with submaximal concentration (EC) of carbachol or histamine. At the end of the experiment, papaverine (500μM) was then added. BNP induced a weak relaxant activity on carbachol-contracted bronchi in nonsensitized (relaxation: 4.23±0.51 %) and passively sensitized bronchi (relaxation: 11.31±2.22 %). On the other hands, BNP induced a relaxant activity on His-contracted bronchi in nonsensitized (relaxation: 42.52±9.03 %) and in passively sensitized (relaxation: 60.57±9.58 %). All these findings are a clear documentation of the modest relaxant role of BNP in asthma and, likely, COPD.

Mots clés: brain natriuretic peptide; carbachol; histamine; human airways; isolated bronchi; passive sensitization

Maria Gabriella Matera, Luigino Calzetta, Vincenzo Parascandolo, Giacomo Curradi, Paola Rogliani, et al.. Relaxant effect of brain natriuretic peptide in nonsensitized and passively sensitized isolated human bronchi. Pulmonary Pharmacology & Therapeutics, 2009, 22 (6), pp.478. ⟨10.1016/j.pupt.2009.04.005⟩. ⟨hal-00589448⟩