Determination of bisphenol A in wine by sol-gel immunoaffinity chromatography, HPLC and fluorescence detection

Zdenka Brenn-Struckhofova (1) , Margit Cichna-Markl (2)

1 Department of Analytical and Food Chemistry
2 Department of Analytical and Food Chemistry

The paper presents a highly selective analysis method for the determination of traces of BPA in wine and the results of its application to 59 wine samples sourced from vats (steel, wood and plastic), glass bottles and Tetra Briks. The procedure consists of sample clean-up by sol-gel immunoaffinity chromatography followed by determination of BPA by high performance liquid chromatography and fluorescence detection. The method has a LOD (S/N=3) of 0.1 ng/ml and a LOQ (S/N=6) of 0.2 ng/ml. In 13 of 59 wine samples the BPA concentration was below the LOQ The mean and median for all wine samples with BPA concentrations above the LOQ were 0.58 and 0.40 ng/ml, respectively. These values – the first set of data on BPA in wine - are far lower than previously published BPA levels derived from migration experiments using wine simulants. Experiments carried out by submerging plastic stoppers in ethanol-water (11:89, v/v) up to 11 weeks indicated that detectable amounts of BPA can be leached from some stoppers.

Mots clés: Life Sciences

Zdenka Brenn-Struckhofova, Margit Cichna-Markl. Determination of bisphenol A in wine by sol-gel immunoaffinity chromatography, HPLC and fluorescence detection. Food Additives and Contaminants, 2006, 23 (11), pp.1227-1235. ⟨10.1080/02652030600654382⟩. ⟨hal-00577596⟩