Determination of aflatoxin B1 in tiger nut based soft drinks

Isabel Arranz Hernandez (1) , Joerg Stroka (1) , Michael Neugebauer (2)

1 FSQ - Food Safety and Quality Unit [Geel]
2 Pharmazeutisches Institut

An analytical method for the determination of Aflatoxin B1 (AfB1) in a tiger nut based soft drinks named ‘horchata' is described. The method is based on an immunoaffinity clean-up, followed by HPLC separation and fluorescence detection after electrochemical post-column derivatisation (PCD). The detection limit (S/N = 3) and the quantification limit (S/N = 10) were 0.02 µg/kg and 0.06 µg/kg respectively. The mean recovery at a level of 2 µg/L was 88 % (n = 6) and the coefficient of variation (CV) 9 %. The method was applied to conduct a small market survey for a beverage named ‘horchata' that is frequently consumed by all kind of population in Southern Europe. A total of 22 samples from Spanish and Belgian supermarkets were analysed. As a result only one sample was found to contain AfB1 at the LOQ of the method.

Mots clés: Life Sciences

Isabel Arranz Hernandez, Joerg Stroka, Michael Neugebauer. Determination of aflatoxin B1 in tiger nut based soft drinks. Food Additives and Contaminants, 2006, 23 (03), pp.305. ⟨10.1080/02652030500415652⟩. ⟨hal-00577561⟩