Absorption, distribution and elimination of fumonisin B1 metabolites in weaned piglets
Résumé
The absorption, distribution and elimination of fumonisin B1 (and B2) after oral administration of Fusarium verticillioides (MRC 826) fungal culture, mixed into the experimental feed for 10 days, was studied in weaned barrows. In order to determine the absorption of FB1 from the feed marked by chromium oxide, a special T-cannula was implanted into the distal part of pigs’ ileum. During the feeding of toxin-containing diet (45 mg FB1 per kg) and until the 10th day after the end of treatment, the total quantity of urine and faeces was collected and their toxin content was analysed. At the end of trial lung, liver, kidney, brain, muscle and fat samples were also collected and their fumonisin content was analysed by LC-MS. The fumonisins appeared to decrease the reduced glutathione content in blood plasma and red blood cell haemolysate, possibly associated with in vivo lipid peroxidation. From a dataset of eighty individual data and the concentration and rate of Cr and fumonisins (FB1, partially hydrolysed FB1 and aminopentol) in the chymus, it could be established that the accumulative absorption of fumonisin B1 was 3.9±0.7%. In the chymus, the FB1 conversion into aminopentol and partially hydrolysed FB1 was 1% and 3.9%, respectively. The degree of metabolism in faeces was variable, although the main product was the partially hydrolysed form, with very small amounts of the aminopentol moiety being recovered. In the investigated tissues the FB1 conversion to aminopentol and partially hydrolysed FB1 was 30% and 20%, respectively.
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