Detection of Aspergillus carbonarius and other black aspergilli from grapes by DNA OLISA^TM microarray

Emmanuel Bufflier (1) , Antonia Susca (2) , Maryline Baud (1) , Giuseppina Mulé (2) , Karen Brengel-Pesce (1) , Antonio Logrieco (2)

1 Bio-Mérieux [Marcy l'Etoile]
2 ISPA - Institute of Sciences of Food Production

Black aspergilli, and particularly Aspergillus carbonarius, are responsible for ochratoxin A production in grapes. Correct identification of these species is essential for toxicological risk assessment in grape and wine. Low-complexity oligonucleotide microarray (OLISA™, Apibio, F) based on DNA oligonucleotides probes, obtained from sequences of the calmodulin gene, was set up in order to detect A. carbonarius, A. japonicus/A. aculeatus and A. ibericus isolated from grape. The designed microarray distinguished all Aspergillus species and the detection limit for A. carbonarius was 3.2 pg of DNA, as template for the PCR reaction. The developed microarray offers a quick and parallel analysis to detect individual Aspergillus species in pure cultures and in naturally contaminated grapes samples.

Mots clés: Life Sciences

Emmanuel Bufflier, Antonia Susca, Maryline Baud, Giuseppina Mulé, Karen Brengel-Pesce, et al.. Detection of Aspergillus carbonarius and other black aspergilli from grapes by DNA OLISA^TM microarray. Food Additives and Contaminants, 2007, 24 (10), pp.1138-1147. ⟨10.1080/02652030701579447⟩. ⟨hal-00577315⟩