Validation of two enzyme immunoassays for aminoglycoside residues according to European Decision 657/2002

Francesca Diana (1) , Maurizio Paleologo (1) , Lidija Persic (1)

1 Tecna Srl

Aminoglycoside antibiotics are commonly used in the treatment of bacterial infections in human and veterinary practice. Because of their toxicity, European Community has established Maximum Residue Limits (MRL) in foodstuffs of animal origin (EEC No 2377/90). In the present work the performances of two new enzyme immunoassays (EIA), I'screen Gentamicin and I'screen Neomycin, for the quantitative detection of the aminoglycosides gentamicin and neomycin in milk and tissue are described. The validation of these EIAs has been performed in accordance to criteria of the European Decision 657/2002. Assays sensitivity at the MRLs was 95% for milk samples and 100% for tissue samples, while specificity was 100% at 33% and 25% of the MRLs for milk and tissues, respectively. The performance of these EIAs indicates that they can be used as easy screening methods in the analysis of aminoglycosides in milk and tissue samples.

Mots clés: Life Sciences

Francesca Diana, Maurizio Paleologo, Lidija Persic. Validation of two enzyme immunoassays for aminoglycoside residues according to European Decision 657/2002. Food Additives and Contaminants, 2007, 24 (12), pp.1345-1352. ⟨10.1080/02652030701458097⟩. ⟨hal-00577294⟩