Silicalites and Mesoporous Silica Nanoparticles for photodynamic therapy

Ouahiba Hocine (1) , Magali Gary-Bobo (2) , David Brevet (1) , Marie Maynadier (2) , S. Fontanel , Laurence Raehm (1) , Sébastien Richeter (1) , Bernard Loock (3) , Pierre Couleaud , Céline Frochot (4, 5) , Clarence Charnay (1) , Gaëlle Derrien (1) , Monique Smaihi (6) , A. Sahmoune , Alain Morère (7) , Philippe Maillard (3) , Marcel Garcia (2) , J.O. Durand (1)

1 ICGM ICMMM - Institut Charles Gerhardt Montpellier - Institut de Chimie Moléculaire et des Matériaux de Montpellier
2 IRCM - U896 Inserm - UM1 - Institut de recherche en cancérologie de Montpellier
3 CSVB - Conception, synthèse et vectorisation de biomolécules
4 PHOTOMED - Médicaments Photoactivables - Photochimiothérapie
5 LRGP - Laboratoire Réactions et Génie des Procédés
6 IEM - Institut Européen des membranes
7 IBMM - Institut des Biomolécules Max Mousseron [Pôle Chimie Balard]

The synthesis of silicalites and Mesoporous Silica Nanoparticles (MSN), which covalently incorporate original water-soluble photosensitizers for PDT applications is described. PDT was performed on MDA-MB-231 breast cancer cells. All the nanoparticles showed significant cell death after irradiation, which was not correlated with 1O2 quantum yield of the nanoparticles. Other parameters are involved and in particular the surface and shape of the nanoparticles which influence the pathway of endocytosis. Functionalization with mannose was necessary to obtain the best results with PDT due to an active endocytosis of mannose-functionalized nanoparticles. The quantity of mannose on the surface should be carefully adjusted as a too high amount of mannose impairs the phototoxicity of the nanoparticles. Fluorescein was also encapsulated in MCM-41 type MSN in order to localize the nanoparticles in the organelles of the cells by confocal microscopy. The MSN were localized in lysosomes after active endocytosis by mannose receptors.

Domaines: Matériaux

Ouahiba Hocine, Magali Gary-Bobo, David Brevet, Marie Maynadier, S. Fontanel, et al.. Silicalites and Mesoporous Silica Nanoparticles for photodynamic therapy. International Journal of Pharmaceutics, 2009, 402 (1-2), pp.221-230. ⟨10.1016/j.ijpharm.2010.10.004⟩. ⟨hal-00575771⟩