Identification of novel microsatellite loci in the sand martin, , and cross-amplification of loci from other bird species

Wouter F. D. Dongen (1, 2) , Gopi K. Munimanda (1) , Jakob Augustin (3) , Donald Blomqvist (3) , Tibor Szép (4) , Richard H. Wagner (1)

1 Konrad Lorenz Institute for Ethology
2 Departamento de Ciencias Ecológicas, Facultad de Ciencias
3 Department of Zoology
4 Institute of Environmental Sciences [Nyíregyháza]

We isolated and characterised six novel microsatellite loci for paternity analysis in the sand martin , by screening an enriched genomic library. In addition, we tested 16 already published microsatellite markers, five of which were also polymorphic in the sand martin. Only one of these 11 loci exhibited evidence of null alleles, and all were polymorphic (mean  = 0.68, range of number of alleles per locus = 4–24), making them suitable for individual heterozygosity quantification and paternity assessment in this species (exclusion probability of 11 unlinked loci = 0.999997).

Mots clés: Sand Martin; Paternity; Microsatellite; Cross-amplification

Wouter F. D. Dongen, Gopi K. Munimanda, Jakob Augustin, Donald Blomqvist, Tibor Szép, et al.. Identification of novel microsatellite loci in the sand martin, , and cross-amplification of loci from other bird species. Journal für Ornithologie = Journal of Ornithology, 2010, 151 (3), pp.761-764. ⟨10.1007/s10336-010-0494-z⟩. ⟨hal-00574009⟩