A New Generation of Sterile and Radiopaque Impression Materials—an Cytotoxicity Study

Chiara Coppi (1) , Chiara Paolinelli Devincenzi (2) , Sergio Bortolini (1) , Ugo Consolo (1) , Roberta Tiozzo (2)

1 Section of Dentistry
2 Section of General Pathology

Impression materials are largely used to record the geometry of dental tissue. Hence, the assessment of their possible cytotoxicity is a necessary step in the evaluation of their biocompatibility. The present study is carried out to evaluate the cytotoxicity of a new elastomeric sterile and radiopaque impression material. Human gingival fibroblasts, cultured in vitro are exposed directly to Elite Implant in three different viscosities, heavy, medium, and light. At 3, 9, 24, 48, and 72 h, the cellular proliferation is evaluated. In parallel, human gingival fibroblasts are exposed indirectly by means of fluid extracts of Elite Implant. The cellular viability is evaluated by 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide, (MTT) assay (Sigma, St Louis, Mo). The gingival fibroblasts proliferation and viability are unaffected by the presence of Elite Implant. This new impression material may represent a safe medical device for clinical and surgical applications. In addition, this material is radiopaque and, thus, can be identified radiographically.

Mots clés: dental impression material; human gingival fibroblast; in vitro cytotoxicity tests; proliferation; viability.; viability

Chiara Coppi, Chiara Paolinelli Devincenzi, Sergio Bortolini, Ugo Consolo, Roberta Tiozzo. A New Generation of Sterile and Radiopaque Impression Materials—an Cytotoxicity Study. Journal of Biomaterials Applications, 2007, 22 (1), pp.83-95. ⟨10.1177/0885328206071928⟩. ⟨hal-00570775⟩