Carriage of genes for various extended-spectrum β-lactamases: a novel resistance strategy of in Poland

Danuta Dzierżanowska (1) , Wanda Kamińska (1) , Katarzyna Semczuk (1) , Dariusz Borowiec (1) , Michał Matysiak , Anna Szumała-Kąkol (2) , Rafał Gierczyński (3) , Jan A. Patzer (1)

1 Department of Clinical Microbiology and Immunology
2 Poznań University of Medical Sciences
3 Department of Bacteriology

Among 110 randomly sampled strains from a collection of 247 extended-spectrum β-lactamase (ESBL)-producing clinical isolates of collected from hospitalised children in three paediatric hospitals in Poland, 64 strains (58.2%) with multiple ESBLs were found, including five non-clonal strains (4.5%) harbouring genes for ESBLs of three families (CTX-M, SHV and TEM). This is the first report of the emergence of triple ESBL-producing in Poland. In addition, strains harbouring genes for TEM-130 and TEM-132 ESBLs were detected in Poland for the first time. Epidemiological analysis of the multiple ESBL-producing isolates by pulsed-field gel electrophoresis (PFGE) revealed a relatively high genetic diversity between isolates producing the same combination of enzymes. Clonally related strains were uncommon.

Mots clés: Paediatric hospital; Multiple extended-spectrum β-lactamases; ESBLs; Epidemiology; PFGE

Danuta Dzierżanowska, Wanda Kamińska, Katarzyna Semczuk, Dariusz Borowiec, Michał Matysiak, et al.. Carriage of genes for various extended-spectrum β-lactamases: a novel resistance strategy of in Poland. International Journal of Antimicrobial Agents, 2010, 35 (4), pp.392. ⟨10.1016/j.ijantimicag.2009.12.010⟩. ⟨hal-00567284⟩