Male infertility-linked point mutation disrupts the Ca2+ oscillation-inducing and PIP2 hydrolysis activity of sperm PLCζ

Michail Nomikos , Khalil Elgmati , Maria Theodoridou , Brian Calver , Bevan Cumbes , George Nounesis , Karl Swann , F. Anthony Lai (1)

1 Medicine - Cardiology

A male infertility-linked human PLCζ mutation introduced into mouse PLCζ completely abolishes both in vitro PIP2 hydrolysis activity and the ability to trigger in vivo Ca2+ oscillations in mouse eggs. Wild-type PLCζ initiated a normal pattern of Ca2+ oscillations in eggs in the presence of 10-fold higher mutant PLCζ suggesting that infertility is not mediated by a dominant-negative mechanism.

Mots clés: Life Sciences

Michail Nomikos, Khalil Elgmati, Maria Theodoridou, Brian Calver, Bevan Cumbes, et al.. Male infertility-linked point mutation disrupts the Ca2+ oscillation-inducing and PIP2 hydrolysis activity of sperm PLCζ. Biochemical Journal, 2011, 434 (2), pp.211-217. ⟨10.1042/BJ20101772⟩. ⟨hal-00565908⟩